Pharmaceutical companies annually synthesize thousands of compounds in drug discovery laboratories in an attempt to find new drug molecules for commercialization. Each of these synthesized compounds may require many reaction and purification steps for further testing.
The primary technique used in the pharmaceutical industry to purify these compounds is flash chromatography and it is preferred because of high sample loading capacity and ease of use.
Unfortunately, existing flash chromatography instrumentation suffers from poor peak resolution and uses large volumes of organic solvents as the mobile phase in the separation. Within medicinal chemistry laboratories, flash chromatography is the main source of chemical waste.
Applied Separations’ liquid CO2 flash chromatography system will fill an unmet need in the pharmaceutical industry to more rapidly purify samples and reduce toxic solvent consumption in the drug discovery process. The technology can be used in medicinal chemistry laboratories, contract research laboratories, and university pharmaceutical research laboratories in the United States and worldwide.
Significantly lower solvent consumption
Lower solvent waste
Quicker and easier recovery of separated compounds
Increased loading capacity
Greater flexibility in separation columns
- Use smaller particle sizes
- Different bonded phases
Multiple sample introduction formats:
- Dry loading onto silica
- Liquid loading onto disposable column
- Direct liquid loading onto a
Control of both carbon dioxide pressure and flow rate
Control of modifier solvent flow rate
Disposable separation columns OR
Performance separation columns packed with smaller particles
Automated system with a user friendly
interface and software
Open bed fraction collector
- Time based fraction collection
- Peak triggered fraction collection
Environmentally Friendly Green Flash With Outstanding Performance
eCO2Chrom - a competitively priced liquid CO2 flash chromatography system that will rapidly separate compounds with high resolution and significantly reduce organic solvent consumption.
A chromatogram of an 8 component mix contain a wide variety of compounds including both acidic and basic chemicals. This chromatogram is an example of separation scope of the instrument and the liquid CO2 separation technique.
A chromatogram showing the separation of Ibuprofen and Ketoprofen. These compounds have similar
chemical structures and show the
selectivity that we can achieve with the
eCO2Chrom CO2 Flash instrument.
A chromatogram of a 400µg acetaminophen sample injected directly onto a cartridge column. This chromatogram shows the detection sensitivity and most importantly the high degree of baseline stability that we can achieve with the eCO2Chrom CO2 Flash instrument.
Research reported here was supported in part (25%) by a Phase 2 Small Business Innovative Research (SBIR) grant from the National Institutes of Health under award number: 5R44GM122107-02
The content is solely the responsibility of Applied Separations, Inc. and does not necessarily represent the official views of the National Institutes of Health.