SPE Cartridges

Polymeric Packings: RP and XAD Resins

Reverse Phase and XAD Polymer-based Resins for Sample Preparation

• Reverse Phase Resins
  Spe-ed RP resins are a family of polymeric media useful for sample preparation. Spe-ed RP resins are porous spherical beads, contain a high surface area and are chemically stable to maximize extraction efficiency.
• Amberlite® XAD Resins
  XAD Resins are neutral, macroporous polymeric beads and derive their adsorptive characteristics from their macroreticular porosity, pore size distribution, high surface area and chemically homogeneous non-ionic structure. These resins are primarily used to extract non-polar, hydrophobic compounds from aqueous or polar solutions.

Reverse Phase Resins

Large bio molecules are not adsorbed onto the RP resin surface. Because of their larger size, they are excluded from adsorbing onto the active sorbent site inside the pores. Smaller molecules are trapped on the active sorbent sites in these pores.

Substitute for C18 SPE Cartridges

Spe-ed RP resin cartridges offer many advantages.

Directly substitute Spe-ed RP resins in your current applications where silica-based C18 may not be giving you optimum recovery.

• pH stable (1 - 14)
  • process high and low pH samples without sorbent decomposition like C18
  • retain strongly basic amines at high pH conditions



• Eliminate residual silanols
  • eliminate secondary interaction caused by residual silanols on C18/silica SPE cartridges (use exclusively reverse phase mechanism)


• Biomolecules to small drugs
  • the range of pore sizes allows the application of Spe-ed RP cartridges for many types of molecules from small molecule drugs to oligonucleotides
  • optimized particle size for easy throughput of biological samples such as urine, plasma or serum


• Applications
  • replace C18/silica SPE cartridges with pH resistant Spe-ed RP resins
  • remove endotoxins
  • exchange solvents
  • separate hydrophobic molecules by size
  • desalt samples
  • environmental - herbicide / pesticide residues
  • sulfonylurea herbicides

RP Resin Characteristics

RP resins are non-polar, non-ionic, ultra-clean, highly crossed-linked polymers that differ in particle size, pore size and surface area. Select a resin with a pore size large enough to allow your analyte to enter the pore and adsorb onto the pore surface.

	PARTICLE SIZE (AVG) microns	EXCLUSION LIMIT daltons
RP104	35	800,000
RP103	75	300,000
RP102	100	400,000
RP101	120	70,000

Resin Selection

Spe-ed RP cartridges are pre-packed with measured amounts of dry, free-flowing, chromatographic resin. The cartridges are made of medical grade polypropylene. These pre-filled cartridges are available in a wide range of sorbent weight and cartridge size combinations.

Standard Sizes

	50mg/1mL	100mg/1mL	200mg/3mL	500mg/6mL
RP101	4295	4201	4202	4204
RP102	4299	4207	4208	4210
RP103	4296	4213	4214	4216
RP104	4297	4219	4220	4222
cartridges/ box	100	100	50	30

     Custom resin weights available.

Amberlite® XAD Resins

• Precleaned, Prepacked, Preconditioned
  Amberlite® XAD resins are neutral, macroporous polymeric beads and derive their adsorptive characteristics from their macroreticular porosity, pore size distribution, high surface area and chemically homogeneous non-ionic structure. These resins are primarily used to extract non-polar, hydrophobic compounds from aqueous or polar solutions.
  
• Specially Prepared - Cleaned
  Spe-ed^TM cartridges are prefilled with measured amounts of specially prepared and cleaned XAD resins for critical analytical use. The polypropylene cartridges are available in 1mL, 3mL, 6mL, 12mL, 30mL, and 60mL sizes. Various amounts of analytical resin (100mg to 20g) are packed in the cartridge between an upper and lower 20µm polyethylene disc.
  
• Pre-swollen - Eliminate Variability
  Commercial XAD resins are normally not suitable for analytical or other applications requiring high purity. Applied Separations’ XAD products are given a proprietary multiple solvent wash to assure the highest level of quality. Variability problems are eliminated with pre-swollen, preconditioned beads. To ensure continuing quality, the cartridge with the XAD resin is then capped and sealed to maintain the resin in its hydrated form.
  
• Resin Selection
  XAD resins differ in pore size, surface area and chemical composition. Select a resin with a pore size large enough to allow your analyte to enter the pore and adsorb onto the pore surface.

Applications
We have compiled a comprehensive list of SPE and Flash chromatography applications available for free. Click here to view the list of the the applications you can put to use immediately in your lab.